Aloe vera for field plantation was achieved from shoot tip explants cultured on MS medium supplemented with BAP (0.9 mg/l), NAA (0.4 mg/l) and citric acid (10 mg/l). Citric acid (10 mg/l) has been added to the medium to alleviate the effect of browning in Aloe. A combination of BA and NAA enhanced the multiple shoots proliferation from shoot tip culture. Maximum shoot multiplication (5-10 shoots per explant) was obtained in 3rd subculture as compared to previous studies. In vitro regenerated shoots also demonstrated root induction on MS medium supplemented with NAA (0.4 mg/l). The plantlets so generated were processed through hardening procedure for acclimatization with greenhouse facilities and transfer to the soil. Through this procedure about 30 plantlets were generated from one explant over three subcultures (total 3 months). The in vitro raised plants were similar to the mother plant in general morphology.
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